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versadoc tm mp 4000 molecular imager ® system  (Bio-Rad)


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    Bio-Rad versadoc tm mp 4000 molecular imager ® system
    The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM <t>MP</t> <t>4000</t> Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.
    Versadoc Tm Mp 4000 Molecular Imager ® System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/versadoc+tm+mp+4000+imager/Image+Lab+Touch+Software+for+ChemiDoc+Touch+Imager+Version+2%2E3/pmc08467447-162-27-35
    Average 90 stars, based on 1 article reviews
    versadoc tm mp 4000 molecular imager ® system - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "An Influence of Modification with Phosphoryl Guanidine Combined with a 2′-O-Methyl or 2′-Fluoro Group on the Small-Interfering-RNA Effect"

    Article Title: An Influence of Modification with Phosphoryl Guanidine Combined with a 2′-O-Methyl or 2′-Fluoro Group on the Small-Interfering-RNA Effect

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22189784

    The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.
    Figure Legend Snippet: The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.

    Techniques Used: Labeling, Modification

    Cleavage site determination in FITC-labeled modified siRNAs by denaturing PAGE. Electropherograms for products of degradation of five siRNAs after 7.5 µg/mL RNAse A treatment. Each image has a D lane representing an RNAse A-treated duplex that contains S-OMe* and various derivatives of the antisense chain ( As-F* , As-F, As-OMe* , As-OMe , or As ). The products of degradation of FITC-labeled siRNA duplexes were visualized in the gel via scanning and recording of an image by means of the VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 are the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of the FITC-labeled As with RNase T1, respectively. The asterisk (*) means the PG-modified chain.
    Figure Legend Snippet: Cleavage site determination in FITC-labeled modified siRNAs by denaturing PAGE. Electropherograms for products of degradation of five siRNAs after 7.5 µg/mL RNAse A treatment. Each image has a D lane representing an RNAse A-treated duplex that contains S-OMe* and various derivatives of the antisense chain ( As-F* , As-F, As-OMe* , As-OMe , or As ). The products of degradation of FITC-labeled siRNA duplexes were visualized in the gel via scanning and recording of an image by means of the VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 are the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of the FITC-labeled As with RNase T1, respectively. The asterisk (*) means the PG-modified chain.

    Techniques Used: Labeling, Modification

    Related Articles

    other:

    Article Title: Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts
    Article Snippet: The fluorescent bands were visualized and quantified using a VersaDoc TM MP 4000 Imager (Bio-Rad).



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    The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM <t>MP</t> <t>4000</t> Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.
    Versadoc Tm Mp 4000 Molecular Imager ® System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/versadoc+tm+mp+4000+imager/Image+Lab+Touch+Software+for+ChemiDoc+Touch+Imager+Version+2%2E3/pmc08467447-162-27-35
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    versadoc tm mp 4000 molecular imager ® system - by Bioz Stars, 2026-09
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    The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM <t>MP</t> <t>4000</t> Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.
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    versadoc tm imaging system 4000 mp  (Bio-Rad)
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    The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM <t>MP</t> <t>4000</t> Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.
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    The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM <t>MP</t> <t>4000</t> Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.
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    The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM <t>MP</t> <t>4000</t> Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.
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    Image Search Results


    The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.

    Journal: International Journal of Molecular Sciences

    Article Title: An Influence of Modification with Phosphoryl Guanidine Combined with a 2′-O-Methyl or 2′-Fluoro Group on the Small-Interfering-RNA Effect

    doi: 10.3390/ijms22189784

    Figure Lengend Snippet: The products of siRNA duplex degradation after 7.5 µg/mL RNAse A treatment, according to denaturing PAGE. ( A ). The black arrow indicates cleavage sites C 4 identified in an FITC-labeled antisense strand; red arrow: U 12 in an FITC-labeled antisense strand; green arrow: the C 16 or U 17 site in an FITC-labeled antisense strand; blue arrow: U 18 in the unlabeled sense strand of the duplex. ( B – F ): Gel images of 20% PAAGs with corresponding FITC-labeled antisense chains; +RNase A : duplexes treated with Rnase A; -RNAse A : control (undigested) duplexes. Each sample contained 32 P-labeled and FITC-labeled antisense chains of the same structure (1.5 and 50 pmol, respectively) and 55 pmol of an unlabeled sense chain. Antisense strands are designated at the bottom of each image ( B: As, C: As-Ome, D: As-Ome*, E: As-F, and F: As-F* ). Lane 1 contains a native sense ( S ) chain in all images. Other lanes contain a modified sense chain: Lane 2 : S-F, Lane 3: S-F*, Lane 4 : S-Ome , and Lane 5 : S-Ome *. The products of degradation of FITC-labeled siRNAs duplexes were visualized in the gel via scanning and recording of an image using a VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 present the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of FITC-labeled As with Rnase T1, respectively. The asterisk (*) means the PG-modified chain.

    Article Snippet: After electrophoretic separation, the products of degradation of the FITC-labeled siRNA duplexes were visualized in the gel by scanning and recording an image by means of the VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm.

    Techniques: Labeling, Modification

    Cleavage site determination in FITC-labeled modified siRNAs by denaturing PAGE. Electropherograms for products of degradation of five siRNAs after 7.5 µg/mL RNAse A treatment. Each image has a D lane representing an RNAse A-treated duplex that contains S-OMe* and various derivatives of the antisense chain ( As-F* , As-F, As-OMe* , As-OMe , or As ). The products of degradation of FITC-labeled siRNA duplexes were visualized in the gel via scanning and recording of an image by means of the VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 are the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of the FITC-labeled As with RNase T1, respectively. The asterisk (*) means the PG-modified chain.

    Journal: International Journal of Molecular Sciences

    Article Title: An Influence of Modification with Phosphoryl Guanidine Combined with a 2′-O-Methyl or 2′-Fluoro Group on the Small-Interfering-RNA Effect

    doi: 10.3390/ijms22189784

    Figure Lengend Snippet: Cleavage site determination in FITC-labeled modified siRNAs by denaturing PAGE. Electropherograms for products of degradation of five siRNAs after 7.5 µg/mL RNAse A treatment. Each image has a D lane representing an RNAse A-treated duplex that contains S-OMe* and various derivatives of the antisense chain ( As-F* , As-F, As-OMe* , As-OMe , or As ). The products of degradation of FITC-labeled siRNA duplexes were visualized in the gel via scanning and recording of an image by means of the VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm. Lanes LB and T1 are the stochastic hydrolysis in 50 mM NaHCO 3 –Na 2 CO 3 buffer (pH 9.5) and partial digestion of the FITC-labeled As with RNase T1, respectively. The asterisk (*) means the PG-modified chain.

    Article Snippet: After electrophoretic separation, the products of degradation of the FITC-labeled siRNA duplexes were visualized in the gel by scanning and recording an image by means of the VersaDoc TM MP 4000 Molecular Imager ® System (Bio-Rad) after laser excitation at 488 nm.

    Techniques: Labeling, Modification